Aquatic Toxicology and Ecosystem Remediation (Ecotoxicology) Laboratory

General Info

Service/Purpose

Provides baseline and time-sensitive data to investigate issues such as the bioaccumulation of contaminants in food webs, effects of pharmaceutical and sewage exposure on fish endocrine systems, and the cumulative effects of diverse human activities in watersheds on ecosystem health.

Location

University of Waterloo

Facility Lead

Mark Servos

Primary Contacts

Name	Role	Contact Information
Mark Servos	Facility Lead	Email: mservos@uwaterloo.ca Phone: 519-888-4567 x36034
Leslie Bragg	Primary Contact	E-mail: leslie.bragg@uwaterloo.ca Phone: 519-888-4567 x37095

Fee for Service

Lab Equipment & Services

Deployable Systems

Ref	Item	Equipment Purpose	Unit	Internal Rate *	External Rate **	Commercial Rate ***
ATERL-ML-1	Bio-Rad CFX-96 Realtime PCR (x2)	Used to run plates for quantifying gene expression and covid-19 in wastewater	per sample	$600	$690	$930
ATERL-ML-2	Automated plate loader	Loads plates for analysis in the 96 touch	per day	$50	$58	$78
ATERL-ML-3	Bio-Rad CFX- OPUS Realtime PCR (x2)	Used to run plates for quantifying gene expression and covid-19 in wastewater	per sample	$600	$690	$930
ATERL-ML-4	Qiagen Digital PCR	Quantifies nucleic acids	per sample	$800	$920	$1,240
ATERL-ML-5	PCR workstation	Used to prepare reagents for PCR and plate them	per day	$50	$58	$78
ATERL-ML-6	Bio-Rad T100 thermocycler	Used for PCR gene expression	per sample	$100	$115	$155
ATERL-ML-7	Biological safety cabinet	Used to prepare, extract samples for various projects	per day	$50	$58	$78
ATERL-ML-8	NIMBUS automated liquid handler	Plates mastermix and samples for covid-19 and other projects	per sample	$600	$690	$930
ATERL-ML-9	Nanodrop (Thermofisher NanoDrop microvolume spectrophotometers)	Measures total RNA, DNA and proteins for various measures including covid-19 in wastewater	per sample	$100	$115	$155
ATERL-ML-10	Bio-Rad plate sealer	Seals plates to be run with qPCR	per plate	$10	$12	$16
ATERL-ML-11	Refrigerated centrifuges	Used to centrifuge samples that require refrigeration for covid- 19 in wastewater	per day	$50	$58	$78
ATERL-ML-12	MaxQ 6000 incubator	Incubation of yeast cells for determination of total erogenicity of wastewater (YES assay)	per day	$100	$115	$155
ATERL-ML-13	Flake ice machine	Keeps PCR samples cold	per day	$20	$23	$31
ATERL-ML-14	Molecular Devices M3 spectrophotometer plate reader	Many uses. ELISAs, YES assay, other cell cultures	per day	$100	$115	$155
ATERL-ML-15	Perkin Elmer 3110 Tricarb liquid scintillation counter	Radiation measurements. H3, C13, I125 etc.	per sample	$50	$58	$78
ATERL-ML-16	QIAcube Connect	Extracts RNA/DNA for various kits	per day	$200	$230	$310
ATERL-ML-17	Laminar flow hood	Used to prepare and extract samples for YES assay	per day	$50	$58	$78
ATERL-ML-18	Small Eppendorf refrigerated centrifuge	Extraction of RNA/DNA samples for covid-19	per day	$10	$12	$16
ATERL-ML-19	Sonicator	Cleaning glassware	---	---	---	---
ATERL-ML-20	Various microscopes	Otolith age determination, histology, cell culture	per day	$50	$58	$78
ATERL-ML-21	Supelco manual SPE manifolds	Extraction of PPCPs from water samples	per day	$50	$58	$78
ATERL-ML-22	Homogenizers for tissue	Homogenizes tissue samples prior to extraction	per day	$10	$12	$16
ATERL-ML-23	pH meters	Measures pH	per day	$20	$23	$31
ATERL-ML-24	Conductivity meters	Measures conductivity	per day	$20	$23	$31
ATERL-ML-25	DO meters	Measures DO	per day	$20	$23	$31
ATERL-ML-26	YSI handheld multimeters	Measures water quality parameters in stream and in aquatic facility for various projects	per day	$50	$58	$78
ATERL-ML-27	Small centrifuges	Used to centrifuge samples for RNA/DNA extraction and PCR. Measures hemaocrit	per day	$30	$35	$47
ATERL-ML-28	Vortex mixers	Used to shake samples for preparing samples for extraction	per day	$10	$12	$16
ATERL-ML-29	Waterbaths	Used for incubating samples as needed	per day	$10	$12	$16
ATERL-ML-30	Orbital shakers	Mixing and shaking of various samples. Used with YES assay	per day	$10	$12	$16
ATERL-ML-31	Hotplates	Used to keep samples/reagents at certain temperatures for various protocols	per day	$10	$12	$16
ATERL-ML-32	Metler Toledo analytical balance	Measuring analytical standards	per day	$50	$58	$78
ATERL-ML-33	Sartorious analytical balance	Measuring analytical standards	per day	$50	$58	$78
ATERL-ML-34	Lab balances of various sizes	Used to measure weight of chemicals, tissues, organisms, etc.	per day	$30	$35	$47
ATERL-ML-35	Dionex SE500 Nitrogen evaporator	Evaporates samples to dryness before reconstitution	per day	$50	$58	$78
ATERL-ML-36	Dionex Autotrace for SPE extractions	Automated SPE for PPCPs and hormones	per day	$200	$230	$310
ATERL-ML-37	Dionex ASE for solid extractions	Automated accelerated solvent extraction of solids like fish tissue for PPCP analysis	per day	$200	$230	$310
ATERL-ML-38	Dionex Rocket evaporator	Evaporation of samples	per day	$50	$58	$78
ATERL-ML-39	Agilent 2200 Tapestation	Measures RNA/DNA quality	per sample	$100	$115	$155
ATERL-ML-40	Agilent 1260 LC with UV detector and fraction collector	Separates a variety of chemicals for toxicity identification evaluation (TIE) studies. Collects specific fractions/peaks of individual chemicals.	per sample	$500	$575	$775
ATERL-ML-41	Agilent 5975 GC and 6890 MS	Analysis of volatile analytes like PAHs	per sample	$800	$920	$1,240
ATERL-ML-42	Agilent 1260 LC with Agilent 6460 Triple Quad MS	Analysis of PPCP, hormones, drugs of abuse	per sample	$1,200	$1,380	$1,860
ATERL-ML-43	Cytation plate reader	Plate reader with imaging capabilities used for cell culture	per sample	$300	$345	$465

Ref	Item	Deployed At	Unit	Internal Rate *	External Rate **	Commercial Rate ***
ATERL-DS-1	trailer, Field portable trailer (16 ft)	Various field sites as needed	per day	$100	$115	$140
ATERL-DS-2	trailer, 30 ft portable exposure trailer (Atco)	Various field sites as needed	per day	$200	$230	$280
ATERL-DS-3	truck, Ford F-250 super cab truck	Various field sites as needed	per day (+ per km)	$50 (+$0.59)	$58 (+$2)	$70 (+$1)
ATERL-DS-4	truck, Ford F-150 crew cab truck	Various field sites as needed	per day (+ per km)	$50 (+$0.59)	$58 (+$2)	$70 (+$1)
ATERL-DS-5	electrofisher, Smith-Root LR-24 Backpack electrofisher unit	Grand River, ON	per day	$180	$207	$252
ATERL-DS-6	electrofisher, Smith-Root LR-20 Backpack electrofisher unit	Boston Creek and Mackenzie Creek (Six Nations)	per day	$180	$207	$252
ATERL-DS-7	electrofisher, Smith-Root Tote Barge electrofisher with 2.5 GPP	Grand River, ON	per day	$300	$345	$420
ATERL-DS-8	boat, Smith-Root 16ft Electrofishing boat with 5.0 GPP and 50 hp motor	Grand River, ON (various sites)	per day	$2,500	$2,875	$3,500
ATERL-DS-9	boat, Sylvan Alaskan 15 ft boat with 25 hp motor	Grand River, ON (various sites)	per day	$100	$115	$140
ATERL-DS-10	boat, Jon boat with 9.9 hp motor and 3.5 hp kicker	Grand River, ON (various sites)	per day	$100	$115	$140
ATERL-DS-11	boat, 20 ft aluminum working boat (Lady Jane) with two 40 hp motors	Grand River, ON (various sites)	per day	$100	$115	$140
ATERL-DS-12	sonde, YSI Quattro handheld multiparameter (pH, electrical conductivity, temperature, ammonia/nitrite) sonde	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$50	$58	$70
ATERL-DS-13	Water temperature logger (HOBO)	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$20	$23	$28
ATERL-DS-14	Water conductivity logger (HOBO)	Grand River, ON (various sites)	per day	$30	$35	$42
ATERL-DS-15	field balances, A&D Company Field balances 0.001g (FX300i) and 0.01g (FX2000i)	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$20	$23	$28
ATERL-DS-16	turbidity meter, LaMotte 2020i Turbidity meter	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$20	$23	$28
ATERL-DS-17	flow meter, Swoffer 3000 flow meter	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$20	$23	$28
ATERL-DS-18	net, CABIN nets and various other invertebrate kick nets	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$20	$23	$28
ATERL-DS-19	water sampler, Kemmerer water sampler	Grand River, ON (various sites)	per day	$20	$23	$28
ATERL-DS-20	dredge, Ekman dredge	Grand River, ON (various sites)	per day	$20	$23	$28
ATERL-DS-21	net, Plankton net	Grand River, ON (various sites)	per day	$20	$23	$28
ATERL-DS-22	sampler, Serber sampler	Grand River, ON (various sites)	per day	$20	$23	$28
ATERL-DS-23	peristaltic pump, Peristaltic pumps for eDNA sampling (Masterflex and Spectral Field Pro)	Grand River, ON (various sites) Boston Creek and Mackenzie Creek (Six Nations)	per day	$50	$58	$70
ATERL-DP-24	Minnow trap	Grand River, ON (various sites)	per day	$10	$12	$14
ATERL-DS-25	Seine net	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$20	$23	$28
ATERL-DS-26	centrifuge, Thermo Scientific Heraeus Pico 21 Portable centrifuge for hematocrit measurements	Grand River, ON (various sites)	per day	$50	$58	$70
ATERL-DS-27	safety gear, Safety gear: Waders, boots, electrofishing gloves, life jackets, high visibility vests (various brands and sizes), air bubblers	Grand River, ON (various sites) Boston Creek and Mackenzie Creek (Six Nations)	per day	$30	$35	$42
ATERL-DS-28	microscope, Leica MS5 Dissecting Microscope	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$50	$58	$70
ATERL-DS-29	sampler, ISCO 6712 automated samplers (3)	Wastewater treatment plants (various locations) and/or other various locations	per day	$100	$115	$140
ATERL-DS-30	GPS unit, Garmin eTrex Summit Handheld GPS units	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$20	$23	$28
ATERL-DS-31	range finder	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$20	$23	$28
ATERL-DS-32	survey equipment, Surveyors tape and meter sticks for stream measurements	Grand River, ON Boston Creek and Mackenzie Creek (Six Nations)	per day	$10	$12	$14

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