Environmental Genomics Facility
General Info
Service/Purpose
The EGF offers DNA, RNA, and transcriptomic processing from tissue or environmental samples through to analysis.
Location
University of Windsor
Facility Lead
Aaron Fisk
Primary Contacts
| Name | Role | Contact Information |
| Aaron Fisk | Facility Lead | afisk@uwindsor.ca
(519) 253-3000 Ext. 4740 |
| Shelby Mackie | Technician | shelby.toews@uwindsor.ca
(519) 253-3000 Ext. 3761 |
| Daniel Heath | Faculty Supervisor | dheath@uwindsor.ca
(519) 253-3000 Ext. 3762 |
Fee for Service
Lab Equipment & Services
| Ref | Item | Equipment Purpose | Unit | Internal Rate * | External Rate ** | Commercial Rate *** |
| EGF-ML-1 | Tecan Freedom Evo (liquid handling robot) | The Tecan Freedom Evo is a liquid handling robot capable of moving
objects with one arm and pipetting 96 wells with the MCA. This
liquid handling robot solution is set up to do two consecutive DNA
extractions at the same time. Cost per 96 sample extraction. | per sample | $100.44 | $120.53 | $168.74 |
| EGF-ML-2 | QuantStudio 12K Flex (ThermoFisher) qPCR machine – 96-well
Block | The QuantStudio 12K Flex system is a qPCR machine with three interchangeable blocks. This allows the machine to do 96 well, 384 well and Open Array qPCR reactions all on the same machine. This also includes a liquid handling robot (AccuFill) for loading the Open Array chips. qPCR cost per machine use (doesn’t include consumables). | per plate | $55 | $66 | $92.40 |
| EGF-ML-3 | QuantStudio 12K Flex (ThermoFisher) qPCR machine – 384-well
Block | The QuantStudio 12K Flex system is a qPCR machine with three interchangeable blocks. This allows the machine to do 96 well, 384 well and Open Array qPCR reactions all on the same machine. This also includes a liquid handling robot (AccuFill) for loading the Open Array chips. qPCR cost per machine use (doesn’t include consumables). | per plate | $165 | $198 | $277.20 |
| EGF-ML-4 | QuantStudio 12K Flex (ThermoFisher) qPCR machine - Open Array qPCR cost per custom 10 chips (48 samples per chip). | The QuantStudio 12K Flex system is a qPCR machine with three interchangeable blocks. This allows the machine to do 96 well, 384 well and Open Array qPCR reactions all on the same machine. This also includes a liquid handling robot (AccuFill) for loading the Open Array chips. qPCR cost per machine use (doesn’t include consumables). | per 480 samples | $8,300 | $9,960 | $13,944 |
| EGF-ML-5 | S5 Ion Torrent (ThermoFisher) NGS machine | The Ion Torrent NGS system is a sequencing machine that is able to make base pair reads based on fluctuations in pH. The Ion Torrent system is capable of sequencing long 400-600bp amplicon fragments. The S5 also comes with a liquid handling robot system and the chips can read from 1-130 M bp reads depending on the system/chips. Next Gen Sequencing (Ion Torrent) per chip (400bp) (~17M reads). | per 400 bp chip | $2,343 | $2,812 | $3,9356 |
| EGF-ML-6 | S5 Ion Torrent (ThermoFisher) NGS System | The Ion Torrent NGS system is a sequencing machine that is able to make base pair reads based on fluctuations in pH. The Ion Torrent system is capable of sequencing long 400-600bp amplicon fragments. The S5 also comes with a liquid handling robot system and the chips can read from 1-130 M bp reads depending on the system/chips. Next Gen Sequencing (Ion Torrent) per chip (400bp) (~17M reads). | per 600 bp chip | $2,525 | $3,030 | $4,240 |
| EGF-ML-7 | NGS Result Analysis | | per chip | $1,500 | $1,800 | $$2,520 |
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