Environmental Genomics Facility
General Info
Service/Purpose
The EGF offers DNA, RNA, and transcriptomic processing from tissue or environmental samples through to analysis.
Location
University of Windsor
Facility Lead
Aaron Fisk
Primary Contacts
| Name | Role | Contact Information
ROW1 | Aaron Fisk | Facility Lead | afisk@uwindsor.ca^(519) 253-3000 Ext. 4740
ROW2 | Shelby Mackie | Technician | shelby.toews@uwindsor.ca^(519) 253-3000 Ext. 3761
ROW3 | Daniel Heath | Faculty Supervisor | dheath@uwindsor.ca^(519) 253-3000 Ext. 3762
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Fee for Service
Lab Equipment & Services
| Ref | Item | Equipment Purpose | Unit | Internal Rate * | External Rate ** | Commercial Rate ***
ROW1 | EGF-ML-1 | Tecan Freedom Evo (liquid handling robot) | The Tecan Freedom Evo is a liquid handling robot capable of moving^objects with one arm and pipetting 96 wells with the MCA. This^liquid handling robot solution is set up to do two consecutive DNA^extractions at the same time. Cost per 96 sample extraction. | per sample | $100.44 | $120.53 | $168.74
ROW2 | EGF-ML-2 | QuantStudio 12K Flex (ThermoFisher) qPCR machine – 96-well^Block | The QuantStudio 12K Flex system is a qPCR machine with three interchangeable blocks. This allows the machine to do 96 well, 384 well and Open Array qPCR reactions all on the same machine. This also includes a liquid handling robot (AccuFill) for loading the Open Array chips. qPCR cost per machine use (doesn’t include consumables). | per plate | $55 | $66 | $92.40
ROW3 | EGF-ML-3 | QuantStudio 12K Flex (ThermoFisher) qPCR machine – 384-well^Block | The QuantStudio 12K Flex system is a qPCR machine with three interchangeable blocks. This allows the machine to do 96 well, 384 well and Open Array qPCR reactions all on the same machine. This also includes a liquid handling robot (AccuFill) for loading the Open Array chips. qPCR cost per machine use (doesn’t include consumables). | per plate | $165 | $198 | $277.20
ROW4 | EGF-ML-4 | QuantStudio 12K Flex (ThermoFisher) qPCR machine - Open Array qPCR cost per custom 10 chips (48 samples per chip). | The QuantStudio 12K Flex system is a qPCR machine with three interchangeable blocks. This allows the machine to do 96 well, 384 well and Open Array qPCR reactions all on the same machine. This also includes a liquid handling robot (AccuFill) for loading the Open Array chips. qPCR cost per machine use (doesn’t include consumables). | per 480 samples | $8,300 | $9,960 | $13,944
ROW5 | EGF-ML-5 | S5 Ion Torrent (ThermoFisher) NGS machine | The Ion Torrent NGS system is a sequencing machine that is able to make base pair reads based on fluctuations in pH. The Ion Torrent system is capable of sequencing long 400-600bp amplicon fragments. The S5 also comes with a liquid handling robot system and the chips can read from 1-130 M bp reads depending on the system/chips. Next Gen Sequencing (Ion Torrent) per chip (400bp) (~17M reads). | per 400 bp chip | $2,343 | $2,812 | $3,9356
ROW6 | EGF-ML-6 | S5 Ion Torrent (ThermoFisher) NGS System | The Ion Torrent NGS system is a sequencing machine that is able to make base pair reads based on fluctuations in pH. The Ion Torrent system is capable of sequencing long 400-600bp amplicon fragments. The S5 also comes with a liquid handling robot system and the chips can read from 1-130 M bp reads depending on the system/chips. Next Gen Sequencing (Ion Torrent) per chip (400bp) (~17M reads). | per 600 bp chip | $2,525 | $3,030 | $4,240
ROW7 | EGF-ML-7 | NGS Result Analysis | | per chip | $1,500 | $1,800 | $$2,520
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